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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
06/01/2016 |
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Data da última atualização: |
20/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARÃES, A. L. S.; PEREIRA, S. A.; KUSSANO, N. R.; DODE, M. A. N. |
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Afiliação: |
A. L. S. GUIMARÃES, UnB; S. A. PEREIRA, UnB; N. R. KUSSANO, UnB; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
The effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Zygote, p. 1-11, 2015. |
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DOI: |
10.1017/S0967199415000064 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 M) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P < 0.05). When the cilostamide concentration was lowered (10 M) and oocytes were arrested for 8 h, embryo development was improved (P < 0.05) and was similar (P > 0.05) to the control. The deleterious effect of 20 M cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 M) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the |
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Palavras-Chave: |
Cilostamide; Cultura pré-maturação; Meiotic arrest; Oócitos bovinos. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182435/1/effect-of-prematuration-culture-using-phosphodiesterase-type-3-inhibitor-and-insulin-transferrin-and-selenium-on-nuclear-and-cytoplasmic-maturation-of-bovine-oocytes.pdf
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Marc: |
LEADER 01878naa a2200217 a 4500
001 2033071
005 2023-03-20
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1017/S0967199415000064$2DOI
100 1 $aGUIMARÃES, A. L. S.
245 $aThe effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes.$h[electronic resource]
260 $c2015
520 $aThis study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 M) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P < 0.05). When the cilostamide concentration was lowered (10 M) and oocytes were arrested for 8 h, embryo development was improved (P < 0.05) and was similar (P > 0.05) to the control. The deleterious effect of 20 M cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 M) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the
653 $aCilostamide
653 $aCultura pré-maturação
653 $aMeiotic arrest
653 $aOócitos bovinos
700 1 $aPEREIRA, S. A.
700 1 $aKUSSANO, N. R.
700 1 $aDODE, M. A. N.
773 $tZygote, p. 1-11, 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
24/04/2015 |
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Data da última atualização: |
24/04/2015 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MARCON, W. A.; KRABBE, E. L.; SUREK, D.; SCHMIDT, J. M.; RORIG, A. |
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Afiliação: |
WILSON A. MARCON, FACC; EVERTON LUIS KRABBE, CNPSA; DIEGO SUREK, CNPSA; JOICE M. SCHMIDT, Universidade Federal do Paraná - Ca mpus Palotina - PR; ANETE RORIG, Universidade Federal do Paraná - Campus Palotina - PR. |
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Título: |
Avaliação da qualidade da mistura de rações utilizando microtracer® em misturadores verticais. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA, 8., 2014, Concórdia. Anais... Brasília: Embrapa, 2014. p. 25-26. JINC 2014. |
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Idioma: |
Português |
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Palavras-Chave: |
Microtracer®; Misturadores verticais. |
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Thesagro: |
Nutrição. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122724/1/final7655.pdf
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Marc: |
LEADER 00640nam a2200181 a 4500
001 2014129
005 2015-04-24
008 2014 bl uuuu u00u1 u #d
100 1 $aMARCON, W. A.
245 $aAvaliação da qualidade da mistura de rações utilizando microtracer® em misturadores verticais.$h[electronic resource]
260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA, 8., 2014, Concórdia. Anais... Brasília: Embrapa, 2014. p. 25-26. JINC 2014.$c2014
650 $aNutrição
653 $aMicrotracer®
653 $aMisturadores verticais
700 1 $aKRABBE, E. L.
700 1 $aSUREK, D.
700 1 $aSCHMIDT, J. M.
700 1 $aRORIG, A.
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Embrapa Suínos e Aves (CNPSA) |
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